Isolation and characterization of Mycobacterium tuberculosis from extra pulmonary specimens in a tertiary referral hospital of north India
نویسندگان
چکیده
Materials and Methods: A total of 510 different extra pulmonary samples were collected during the period of August 2014 to July 2015. All the samples were subjected to Ziehl Neelsen staining. Samples were decontaminated by NALC-NaOH method and inoculated on a pair of Lowenstein-Jensen (LJ) media and one p-Nitrobenzoic acid (PNB). Cultures were incubated at 37 ̊C and observed for 8weeks for any growth.
منابع مشابه
Mycobacterium tuberculosis Dominance over Nontuberculous Mycobacteria Irrespective of Immune Status: An Indian Scenario
Introduction: In HIV-associated tuberculosis cases, identifying nontuberculous Mycobacteria (NTM) from clinical samples has become essential regarding patients’ treatment and prognosis. This study aims to determine the prevalence of different Mycobacteria species from immunocompromised and immunocompetent patients with suspected tuberculosis attending a tertiary care hospital in Kolkata, India....
متن کاملUtility of Conventional Techniques for Diagnosis of Pulmonary and Extrapulmonary Tuberculosis: Study from a Tertiary Care Hospital in North India
Though pulmonary tuberculosis (PTB) remains the most common presentation, extra pulmonary tuberculosis (EXTB) is another significant clinical problem and due to paucibacillary nature, remains a challenge for diagnosis. The aim of this study is to evaluate the utility of smear microscopy and culture for detection of Mycobacteria in pulmonary and extra pulmonary specimens. This retrospective stud...
متن کاملRapid Screening of MDR-TB in Cases of Extra Pulmonary Tuberculosis Using Geno Type MTBDRplus
BACKGROUND Drug resistance in tuberculosis is a major public health challenge in developing countries. The limited data available on drug resistance in extra pulmonary tuberculosis stimulated us to design our study on anti-tuberculosis drug resistance pattern in cases of extra pulmonary tuberculosis in a tertiary referral hospital of North India. We performed Geno Type MTBDRplus assay in compar...
متن کاملMolecular Identification of Mycobacterium Tuberculosis Complex in Formalin-Fixed, Paraffin-Embedded Tissue Blocks of Extra Pulmonary Speciemens using Genomics Extraction
Background: Tuberculosis has been detected in some extra pulmonary ecological niches. Although extra pulmonary tuberculosis (EPTB) is less frequent than Pulmonary Tuberculosis (PTB), its incidence has increased worldwide. The aim of this study was to investigate the presence of EPTB and MDR-EXPT in Formalin-fixed, paraffin-embedded tissue blocks among different samples in Kerma...
متن کاملحساسیت MTB-PCR خون در تشخیص مایکوباکتریوم توبرکلوزیس
Background: Tuberculosis is still one of the most important causes of mortality and morbidity in many countries and is the second only to human immunodeficiency virus as a cause of death worldwide resulting from a single infectious agent. In 1993, the World Health Organization declared tuberculosis a global public health emergency. Conven-tional methods for the diagnosis of Mycobacterium tuberc...
متن کامل